HABP2 Polyclonal antibody

HABP2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12863-1-AP

Synonyms

Factor VII activating protease, FSAP, HABP, HABP2, HGFAL, hyaluronan binding protein 2, PHBP

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Validation Data Gallery

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  IHC staining of human liver cancer using 12863-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12863-1-AP (HABP2 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 12863-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12863-1-AP (HABP2 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 3 publications below

Product Information

12863-1-AP targets HABP2 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HABP2 fusion protein Ag3981
• Full Name: hyaluronan binding protein 2
• Calculated Molecular Weight: 560 aa, 63 kDa
• GenBank Accession Number: BC031412
• Gene Symbol: HABP2
• Gene ID (NCBI): 3026
• RRID: AB_10640521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HABP2 is also named as HGFAL, PHBP and belongs to the peptidase S1 family. It can activate coagulation factor VII and prourokinase and release the vasoactive peptide bradykinin by cleaving kininogen and is an important role in the regulation of the haemostasis system as well as fibroproliferative inflammatory processes (PMID:21655671). HABP2 is present as the 70 kDa inactive single chain precursor in human plasma and transforms to the active two chain form, 50 kDa heavy chain and 27 kDa light chain,and the 50 kDa heavy chain changes to two 26 kDa fragments, and 27 kDa light chain to 17 kDa and 8 kDa fragments, which are all bridged by disulfide linkages, by autocleavage(PMID:11217080).

Publications

Species	Application	Title
human	IHC	Theranostics; Reversing activity of cancer associated fibroblast for staged glycolipid micelles against internal breast tumor cells.; Authors - Yun Zhu
human	IHC	J Assoc Res Otolaryngol; Untargeted Metabolomic Analysis in Endolymphatic Sac Luminal Fluid from Patients with Meniere's Disease; Authors - Li Huang
mouse	IHC	ACS Chem Neurosci; HABP2 Encapsulated by Peripheral Blood-Derived Exosomes Suppresses Astrocyte Autophagy to Exacerbate Neuroinflammatory Injury in Mice with Ischemic Stroke; Authors - Hongcheng Luo