HABP4 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• HABP4 Polyclonal antibody (33317-1-AP)

HABP4 Polyclonal Antibody for WB, ELISA

Cat No. 33317-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Hyaluronan-binding protein 4, IHABP4, IHABP-4, Intracellular hyaluronan-binding protein 4, Ki-1/57 intracellular antigen

• 

  WB analysis using 33317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33317-1-AP (HABP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A-204 cells, DU 145 cells, HepG2 cells, PANC-1 cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33317-1-AP targets HABP4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag39922

  Product name: Recombinant human HABP4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 280-413 aa of NM_014282.3

  Sequence: LEVEEETQVQEMTLDEWKNLQEQTRPKPEFNIRKPESTVPSKAVVIHKSKYRDDMVKDDYEDDSHVFRKPANDITSQLEINFGNLPRPGRGARGGTRGGRGRIRRAENYGPRAEVVMQDVAPNPDDPEDFPALS

• Full Name: hyaluronan binding protein 4
• Calculated Molecular Weight: 46kDa，413aa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_014282.3
• Gene Symbol: HABP4
• Gene ID (NCBI): 22927
• RRID: AB_3742938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5JVS0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HABP4 (hyaluronic acid binding protein 4) is an intracellular regulatory protein, with a molecular weight of about 57 kDa (PMID: 33245729), which is rich in positively charged amino acids and can bind with negatively charged macromolecules such as RNA and hyaluronic acid. It has SUMO binding activity, participates in PML tissue, negatively regulates DNA binding activity, and positively regulates gene expression. In addition, HABP4 has a complex interaction network in cells, and has undergone a variety of post-translational modifications, including serine/threonine phosphorylation, arginine methylation and SUMO.