HACL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HACL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15519-1-AP

Synonyms

2-hydroxyphytanoyl-CoA lyase, 2-hydroxyacyl-CoA lyase 1, 2-HPCL, 2 hydroxyphytanoyl CoA lyase, 2 hydroxyacyl CoA lyase 1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 15519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15519-1-AP

  WB result of HACL1 antibody (15519-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HACL1 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 15519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15519-1-AP

  IP result of anti-HACL1 (IP:15519-1-AP, 4ug; Detection:15519-1-AP 1:300) with HeLa cells lysate 2640 ug.

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat kidney using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat kidney using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15519-1-AP (HACL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: rat liver tissue, human liver tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15519-1-AP targets HACL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HACL1 fusion protein Ag7830
• Full Name: 2-hydroxyacyl-CoA lyase 1
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC001627
• Gene Symbol: HACL1
• Gene ID (NCBI): 26061
• RRID: AB_2279502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.