Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25940-1-AP targets HAL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22985 Product name: Recombinant human HAL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC096097 Sequence: MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLDNEDRLEVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKSGWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHAL Predict reactive species |
| Full Name | histidine ammonia-lyase |
| Calculated Molecular Weight | 657 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC096097 |
| Gene Symbol | HAL |
| Gene ID (NCBI) | 3034 |
| RRID | AB_2880303 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HAL, also named as Histidase and HIS, catalyzes the nonoxidative elimination of the α-amino group of histidine. It is closely related to the important plant enzyme phenylalanine ammonia-lyase. HAL has some isoforms with MW 73 kDa, 65 kDa and 49 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HAL antibody 25940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HAL Polyclonal Antibody (Cat# 25940-1-AP) has 5 total citations published in Autophagy, Cell Death & Disease, FASEB Journal, Neuropsychiatric Disease and Treatment, and Discover Oncology. Research fields span autophagy, ferroptosis in prostate cancer, hepatic one-carbon metabolism in homocystinuria, gut microbiota remodeling in neuropsychiatric disease, and histidine metabolism in hepatocellular carcinoma and cholangiocarcinoma.
| Species | Application | Title |
|---|---|---|
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple | ||
Cell Death Dis Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway | ||
FASEB J Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment | ||
Neuropsychiatr Dis Treat Antidepressant-Like Effects of Mongolian Medical Warm Acupuncture via Remodeling the Gut Microbiota-Metabolite-Barrier Axis in CUMS Rats. | ||
Discov Oncol Histidine ammonia lyase expression characteristics in primary liver cancer: hepatocellular carcinoma and cholangiocarcinoma. |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The reviewer used it for Western Blot on mouse liver tissue at a 1:4000 dilution and reported strong signal with a clean background, describing it as a very good antibody. Overall, the feedback highlights reliable performance and high specificity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (05-20-2022) | Very good antibody. Strong signal and clean background
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