HAO1 Polyclonal antibody

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  WB analysis of mouse liver using 25056-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 25056-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HAO1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25056-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25056-1-AP targets HAO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAO1 fusion protein Ag21884
• Full Name: hydroxyacid oxidase (glycolate oxidase) 1
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC113665
• Gene Symbol: HAO1
• Gene ID (NCBI): 54363
• RRID: AB_2879875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJM8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.