HARS Polyclonal antibody

HARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16375-1-AP

Synonyms

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

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Validation Data Gallery

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  WB analysis of mouse liver using 16375-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16375-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16375-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16375-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16375-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16375-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16375-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 16375-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16375-1-AP (HARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 16375-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HARS antibody 16375-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, human kidney tissue, K-562 cells, mouse brain tissue, mouse heart tissue, mouse lung tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16375-1-AP targets HARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HARS fusion protein Ag9333
• Full Name: histidyl-tRNA synthetase
• Calculated Molecular Weight: 509 aa, 57 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC011807
• Gene Symbol: HARS
• Gene ID (NCBI): 3035
• RRID: AB_2116283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.; Authors - Maria C Passarelli
mouse	WB	Sci Rep; NRF2 activation ameliorates blood-brain barrier injury after cerebral ischemic stroke by regulating ferroptosis and inflammation; Authors - Wei Fan
mouse	WB	Neuroscience; MyD88 inhibition attenuates cerebral ischemia-reperfusion injury by regulating the inflammatory response and reducing blood-brain barrier damage; Authors - Fangchao Jiang