Multi-rAb® HAT1 Multi-Recombinant antibody

• Home
• Products
• Primary Antibodies
• Multi-rAb® HAT1 Multi-Recombinant antibody (RMX00092)

HAT1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00092

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.3.1.48, KAT1

• 

  WB analysis using RMX00092

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00092 (HAT1 antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using RMX00092

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using RMX00092 (HAT1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using RMX00092

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using RMX00092 (HAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using RMX00092

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAT1 antibody (RMX00092) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, NIH/3T3 cells, PC-12 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00092 targets HAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: histone acetyltransferase 1
• Calculated Molecular Weight: 419 aa, 50 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC018682
• Gene Symbol: HAT1
• Gene ID (NCBI): 8520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: O14929
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAT1 (also known as KAT1) is located on human chromosome 2q31.1 and encodes histone acetyltransferase 1. This gene is widely expressed in various tissues, and the protein is predominantly localized nuclear in normal cells. Its primary function is to acetylate newly synthesized histones H4 (Lys5, Lys12) and H2A (Lys5), thereby promoting chromatin assembly and playing a key role in DNA replication, cell cycle progression, and DNA damage repair. This antibody can detect a band around 46 kDa.