HAUS4 Polyclonal antibody

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  WB analysis of human brain using 20104-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20104-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 20104-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20104-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20104-1-AP (HAUS4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HAUS4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20104-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

C14orf94, HAUS augmin-like complex subunit 4

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Tested Applications

• Positive WB detected in: human brain tissue, human placenta tissue, mouse lung tissue, mouse skin tissue
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

20104-1-AP targets HAUS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13717

  Product name: Recombinant human HAUS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-363 aa of BC002554

  Sequence: MASGDFCSPGEGMEILQQVCSKQLPPCNLSKEDLLQNPYFSKLLLNLSQHVDESGLSLTLAKEQAQAWKEVRLHKTTWLRSEILHRVIQELLVDYYVKIQDTNVTSEDKKFHETLEQRLLVTELMRLLGPSQEREIPPLLGLEKADLLELMPLSEDFVWMRARLQQEVEEQLKKKCFTLLCYYDPNSDADSETVKAAKVWKLAEVLVGEQQQCQDAKSQQKEQMLLLEKKSAAYSQVLLRCLTLLQRLLQEHRLKTQSELDRINAQYLEVKCGAMILKLRMEELKILSDTYTVEKVEVHRLIRDRLEGAIHLQEQDMENSRQVLNSYEVLGEEFDRLVKEYTVLKQATENKRWALQEFSKVYR

• Full Name: chromosome 14 open reading frame 94
• Calculated Molecular Weight: 363 aa, 42 kDa
• Observed Molecular Weight: 28 kDa, 37 kDa, 42 kDa
• GenBank Accession Number: BC002554
• Gene Symbol: C14orf94
• Gene ID (NCBI): 54930
• RRID: AB_10639366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H6D7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC; Authors - Qi Gao
human	WB	J Cell Biol; Reconstitution and mechanistic dissection of the human microtubule branching machinery.; Authors - Yaqian Zhang
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	IF	Nat Commun; An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity; Authors - Cornelia Sala