HAVCR1 Polyclonal antibody

HAVCR1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26420-1-AP

Synonyms

CD365, HAVCR, HAVCR 1, HAVCR1, Kidney injury molecule 1, KIM 1, KIM1, T cell membrane protein 1, TIM, TIM 1, TIM1, TIMD 1, TIMD1

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Validation Data Gallery

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  WB analysis of Jurkat using 26420-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26420-1-AP (HAVCR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26420-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26420-1-AP (HAVCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26420-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26420-1-AP (HAVCR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26420-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26420-1-AP (HAVCR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26420-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26420-1-AP (HAVCR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26420-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26420-1-AP (HAVCR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26420-1-AP is HAVCR1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAVCR1 fusion protein Ag23888
• Full Name: hepatitis A virus cellular receptor 1
• Calculated Molecular Weight: 359 aa, 39 kDa
• GenBank Accession Number: BC013325
• Gene Symbol: HAVCR1
• Gene ID (NCBI): 26762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatitis A virus cellular receptor 1 (HAVCR1, also known as TIM-1, KIM-1 or CD365) is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. HAVCR1 acts as a membrane receptor for hepatitis A virus (HHAV) (PMID: 9658108; 8861957). HAVCR1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). HAVCR1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that HAVCR1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).

Publications

Species	Application	Title
mouse	IHC,IF	Drug Deliv; Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury.; Authors - Jing-Bo Hu