HAVCR1 Polyclonal antibody

HAVCR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30948-1-AP

Synonyms

Havcr1, KIM 1, Kim1

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Validation Data Gallery

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  WB analysis using 30948-1-AP

  Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 30948-1-AP (Havcr1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 30948-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (Havcr1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 30948-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (Havcr1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30948-1-AP targets HAVCR1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

• Tested Reactivity: Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: hepatitis A virus cellular receptor 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_173149.2
• Gene Symbol: Havcr1
• Gene ID (NCBI): 286934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatitis A virus cellular receptor 1 (HAVCR1, also known as TIM-1, KIM-1 or CD365) is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. HAVCR1 acts as a membrane receptor for hepatitis A virus (HAV) (PMID: 9658108; 8861957). HAVCR1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). HAVCR1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that HAVCR1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).