HAX1 Monoclonal antibody

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  WB analysis using 67883-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67883-2-Ig (HAX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67883-2-Ig

  WB result of HAX1 antibody (67883-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells.

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  IF Staining of HeLa using 67883-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HAX1 antibody (67883-2-Ig, Clone: 2A12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

HAX1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67883-2-Ig

Clone No.2A12F11

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

FLJ17042, FLJ18492, FLJ93803, HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• CoIP: See 1 publications below

Product Information

67883-2-Ig targets HAX1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27244

  Product name: Recombinant human HAX1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-279 aa of BC014314

  Sequence: MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR

• Full Name: HCLS1 associated protein X-1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014314
• Gene Symbol: HAX1
• Gene ID (NCBI): 10456
• RRID: AB_2918640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00165
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	CoIP	Phytomedicine; Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts; Authors - Tingting Zhang