HAX1 Monoclonal antibody

HAX1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A12F11

Cat no : 67883-2-Ig

Synonyms

FLJ17042, FLJ18492, FLJ93803, HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3

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Validation Data Gallery

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  WB analysis using 67883-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67883-2-Ig (HAX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67883-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HAX1 antibody (67883-2-Ig, Clone: 2A12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Product Information

67883-2-Ig targets HAX1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HAX1 fusion protein Ag27244
• Full Name: HCLS1 associated protein X-1
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 32 kDa
• GenBank accession number: BC014314
• Gene symbol: HAX1
• Gene ID (NCBI): 10456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.