HBA1-Specific Polyclonal antibody

HBA1-Specific Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 19990-1-AP

Synonyms

Alpha globin, HBA1, HBA2, Hemoglobin alpha chain, Hemoglobin subunit alpha, hemoglobin, alpha 1



Tested Applications

Positive WB detected inhuman heart tissue, human spleen tissue
Positive IF detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19990-1-AP targets HBA1-Specific in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hemoglobin, alpha 1
Calculated molecular weight 16 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberNM_000558
Gene symbol HBA1
Gene ID (NCBI) 3039
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.

Protocols

Product Specific Protocols
WB protocol for HBA1-Specific antibody 19990-1-APDownload protocol
IF protocol for HBA1-Specific antibody 19990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols