HBD Polyclonal antibody

HBD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16824-1-AP

Synonyms

Delta globin, HBD, Hemoglobin delta chain, Hemoglobin subunit delta, hemoglobin, delta

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Validation Data Gallery

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  WB analysis of human plasma using 16824-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 16824-1-AP (HBD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 16824-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 16824-1-AP (HBD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16824-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16824-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human blood tissue, human plasma tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16824-1-AP targets HBD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HBD fusion protein Ag10409
• Full Name: hemoglobin, delta
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC070282
• Gene Symbol: HBD
• Gene ID (NCBI): 3045
• RRID: AB_2114476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Front Pharmacol; Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.; Authors - Chenguang Yang
human	WB	Placenta; SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy; Authors - Yuxuan Jiang