HBG1-Specific Monoclonal antibody

HBG1-Specific Monoclonal Antibody for FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B5

Cat no : 66168-1-Ig

Synonyms

Gamma 1 globin, Hb F Agamma, HBG1, HBG1-Specific, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

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Validation Data Gallery

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  IHC staining of human placenta using 66168-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 66168-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 66168-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66168-1-Ig (HBG1-Specific antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using 66168-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human HBG1-Specific (66168-1-Ig, Clone:2A4B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: K-562 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:16000

Sample-dependent, check data in validation data gallery

Product Information

66168-1-Ig targets HBG1-Specific in IHC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hemoglobin, gamma A
• Calculated molecular weight: 16 kDa
• GenBank accession number: NM_000559
• Gene symbol: HBG1
• Gene ID (NCBI): 3047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.