HBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HBP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 11746-1-AP

Synonyms

HBP1, HMG box containing protein 1, HMG box transcription factor 1

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Validation Data Gallery

• 

  WB analysis using 11746-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11746-1-AP

  IP result of anti-HBP1 (IP:11746-1-AP, 4ug; Detection:11746-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: human brain tissue, SGC-7901 cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

11746-1-AP targets HBP1 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HBP1 fusion protein Ag2342
• Full Name: HMG-box transcription factor 1
• Calculated Molecular Weight: 514 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC022329
• Gene Symbol: HBP1
• Gene ID (NCBI): 26959
• RRID: AB_2247970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMG-box protein 1 (HBP1) is a transcriptional repressor, and has a role in cell cycle progression and tumor suppression. Also it's a repressor of the cyclin D1 gene and inhibits the Wnt signaling pathway. Interaction with RB1 enhances it's binding to the H1F0 promoter . Disrupts the interaction between DNA and TCF4. This is a rabbit polyclonal antibody raised against part of C-terminus of HBP1 of human origin.

Publications

Species	Application	Title
human	WB	Cancer Cell; MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state.; Authors - Yulin Li
human,mouse	WB,IHC	Pharmacol Res; Targeting of HBP1/TIMP3 axis as a novel strategy against breast cancer; Authors - Yue Zhou
mouse,human	WB,IHC	Oncogene; miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.; Authors - Yanyu Wei ; KD Validated
human	WB,IHC	Oncogene; CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.; Authors - Baozhu Yi
human	WB	EMBO Rep; The human GID complex engages two independent modules for substrate recruitment.; Authors - Weaam I Mohamed
mouse	WB	J Cell Physiol; Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.; Authors - Chien-Yi Chan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (11-22-2021)

This antibody gave a strong one band signal.

• Applications: Western Blot