HCFC1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• HCFC1 Polyclonal antibody (19358-1-AP)

HCFC1 Polyclonal Antibody for WB, ELISA

Cat No. 19358-1-AP Copied!

Publications (2)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

• 

  WB analysis of HEK-293 using 19358-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 19358-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 19358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for HCFC1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19358-1-AP targets HCFC1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6798

  Product name: Recombinant human HCFC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC063435

  Sequence: MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQ

• Full Name: host cell factor C1 (VP16-accessory protein)
• Calculated Molecular Weight: 209 kDa
• Observed Molecular Weight: 230 kDa, 300 kDa
• GenBank Accession Number: BC063435
• Gene Symbol: HCFC1
• Gene ID (NCBI): 3054
• RRID: AB_10860105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51610
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Host cell factor 1 (HCFC1), also known as HCF-1, is a transcriptional co-regulator with a proteolytic cleavage at the PRO repeats that could be cleaved by O-GlcNAc transferase (OGT) to produce a heterodimeric complex of HCFC1N and HCFC1C subunits (PMID: 23539139). HCFC1 was first identified in herpes simplex virus transcription while it lacks a DNA binding domain. HCFC1 is not a traditional transcription factor, it interacts with multiple protein including transcription factors to regulate many biological processes, such as cell proliferation, migration, cell death and mitochondrial biogenesis (PMID: 25281006). Mutation in HCFC1 was related to an X-linked recessive disease ---cblX with a complex clinical manifestation involved in error of metabolism,  neurocognitive impairment and  craniofacial abnormalities (PMID: 24011988; PMID: 25281006).

Publications

What published studies show

The HCFC1 Polyclonal antibody (Cat# 19358-1-AP) has a total of 2 citations. These appear in Advanced Science and Microbiology and Immunology. The cited research spans fields including cochlear hair cell senescence and autophagy regulation, as well as viral infection mechanisms in neuronal cells, specifically equine herpesvirus-1 susceptibility in differentiated neurons.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Adv Sci (Weinh); RONIN/HCF1-TFEB Axis Protects Against D-Galactose-Induced Cochlear Hair Cell Senescence Through Autophagy Activation; Authors - Yongjie Wei
human,rat	IF	Microbiol Immunol; Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation-dependent.; Authors - Erina Minato