HCK Polyclonal antibody

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HCK Polyclonal Antibody for IHC, ELISA

Cat No. 11600-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

HCK, hemopoietic cell kinase, JTK9, p59 HCK/p60 HCK, Tyrosine protein kinase HCK

• 

  WB analysis of Jurkat using 11600-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11600-1-AP (HCK antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11600-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11600-1-AP (HCK antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11600-1-AP targets HCK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2132

  Product name: Recombinant human HCK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 221-526 aa of BC014435

  Sequence: ELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

• Full Name: hemopoietic cell kinase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014435
• Gene Symbol: HCK
• Gene ID (NCBI): 3055
• RRID: AB_10640577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08631
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-HCK (Hematopoietic Cell Kinase) antibody (Cat# 11600-1-AP) has 8 citations published in Cell Communications & Signaling, Molecular Therapy, Journal of Hazardous Materials, Frontiers in Pharmacology, Food Science & Nutrition, Immunity Inflammation & Disease, Frontiers in Cardiovascular Medicine, and Frontiers in Genetics. Research fields span neuroinflammation after traumatic brain injury, tumor immunotherapy in TNBC, pulmonary silica nanoparticle injury, NLRP3 inflammasome activation, bladder cancer ferroptosis, diabetic nephropathy, atherosclerosis, and IgA nephropathy.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Commun Signal; Hematopoietic cell kinase regulates microglial/macrophage activation to drive neuroinflammation after traumatic brain injury.; Authors - Lei Li
mouse	WB,IHC	Mol Ther; DCC-2036 induces repolarization of TAMs to M1 type and enhances CD8(+) T cell immunity in TNBC.; Authors - Yuxin Liang ; KO Validated
	WB	J Hazard Mater; Immune activation contributes recovery following pulmonary impairments by silica nanoparticles; Authors - Caixia Guo
human,mouse	WB	Front Pharmacol; Hematopoietic Cell Kinase (HCK) Is Essential for NLRP3 Inflammasome Activation and Lipopolysaccharide-Induced Inflammatory Response In Vivo.; Authors - Xiangxi Kong ; KD Validated
human, mouse	WB,IHC,IF	Food Sci Nutr; Dauricine Inhibits Macrophages M2 Polarization and Regulates the Progression and Ferroptosis via HCK/IDO1 in Urinary Bladder Cancer.; Authors - Jie Gong
human	WB,IHC	Immun Inflamm Dis; Characterization of NLRP3 Inflammasome-Associated Hub Genes in the Progression of Diabetic Nephropathy.; Authors - Sheng Zhao