HCN2 Polyclonal antibody, PBS Only

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• HCN2 Polyclonal antibody, PBS Only (30946-1-PBS)

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  WB analysis using 30946-1-AP (same clone as 30946-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30946-1-AP (HCN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using HCN2 antibody (30946-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

HCN2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 30946-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

BCNG2, BCNG-2, Brain cyclic nucleotide-gated channel 2, HAC 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

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Product Information

30946-1-PBS targets HCN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34038

  Product name: Recombinant human HCN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 750-889 aa of NM_001194

  Sequence: LALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL*

• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 60 kDa, 90 kDa, 120 kDa
• GenBank Accession Number: NM_001194
• Gene Symbol: HCN2
• Gene ID (NCBI): 610
• RRID: AB_3086427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL51
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)