HCN2 Recombinant monoclonal antibody

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  WB analysis using 84308-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84308-3-RR (HCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84308-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84308-3-RR against Human HCN2 were performed. The affinity constant is 0.795 nM.

HCN2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84308-3-RR

Clone No.241601E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84308-3-RR targets HCN2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34038

  Product name: Recombinant human HCN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 750-889 aa of NM_001194

  Sequence: LALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL*

• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_001194
• Gene Symbol: HCN2
• Gene ID (NCBI): 610
• RRID: AB_3671849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UL51
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)