Tested Applications
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18370-1-AP targets HCRTR1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12269 Product name: Recombinant human HCRTR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 321-425 aa of BC074796 Sequence: KRVFGMFRQASDREAVYACFTFSHWLVYANSAANPIIYNFLSGKFREQFKAAFSCCLPGLGPCGSLKAPSPRSSASHKSLSLQSRCSISKISEHVVLTSVTTVLP Predict reactive species |
| Full Name | hypocretin (orexin) receptor 1 |
| Calculated Molecular Weight | 425 aa, 48 kDa |
| Observed Molecular Weight | 48-54 kDa |
| GenBank Accession Number | BC074796 |
| Gene Symbol | HCRTR1 |
| Gene ID (NCBI) | 3061 |
| RRID | AB_10858633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HCRTR1 antibody 18370-1-AP | Download protocol |
| WB protocol for HCRTR1 antibody 18370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HCRTR1 Polyclonal antibody (Cat# 18370-1-AP) has 15 citations. Journals include Nature Biotechnology, Advanced Science, Journal of Ethnopharmacology, European Journal of Medicinal Chemistry, CNS Neuroscience & Therapeutics, Drug Design, Development and Therapy, Frontiers in Pharmacology, BBA Molecular Basis of Disease, Experimental Neurology, Brain Research Bulletin, Journal of Pharmacological Sciences, Neuropeptides, and Evidence-Based Complementary and Alternative Medicine. Research fields span neuroscience, sleep/insomnia, orexin signaling, neuroinflammation, traumatic brain injury, depression, pain, and metabolism.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells | ||
Adv Sci (Weinh) Hypocretin-1/Hypocretin Receptor 1 Regulates Neuroplasticity and Cognitive Function through Hippocampal Lactate Homeostasis in Depressed Model | ||
J Ethnopharmacol Regulatory effects of Yixin Anshen Granules on neurotransmitters and receptor expression in insomnia model rodents. | ||
Eur J Med Chem Discovery, asymmetric synthesis and biological evaluation of new bisbenzylisoquinoline-orexin receptor antagonists for insomnia treatment | ||
CNS Neurosci Ther Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury | ||
Drug Des Devel Ther Orexin-A Reverse Bone Mass Loss Induced by Chronic Intermittent Hypoxia Through OX1R-Nrf2/HIF-1α Pathway. |
