HDAC1-specific Polyclonal antibody

HDAC1-specific Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16160-1-AP

Synonyms

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1



Tested Applications

Positive WB detected inmouse testis tissue, mouse brain tissue, mouse thymus tissue
Positive IP detected inmouse testis tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16160-1-AP targets HDAC1-specific in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name histone deacetylase 1
Calculated molecular weight 55 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_004964
Gene symbol HDAC1
Gene ID (NCBI) 3065
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.

Protocols

Product Specific Protocols
WB protocol for HDAC1-specific antibody 16160-1-APDownload protocol
IF protocol for HDAC1-specific antibody 16160-1-APDownload protocol
IP protocol for HDAC1-specific antibody 16160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols