HDAC10 Monoclonal antibody, PBS Only

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  WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67646-1-Ig (same clone as 67646-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67646-1-Ig (same clone as 67646-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

HDAC10 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67646-1-PBS

Clone No.1A1B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

1A1B11, EC:3.5.1.48, HD 10, HD10, HDAC 10

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Product Information

67646-1-PBS targets HDAC10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18470

  Product name: Recombinant human HDAC10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 320-669 aa of BC125083

  Sequence: TLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA

• Full Name: histone deacetylase 10
• Calculated Molecular Weight: 669 aa, 71 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC125083
• Gene Symbol: HDAC10
• Gene ID (NCBI): 83933
• RRID: AB_2882846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q969S8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.