|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, human kidney tissue, Jurkat cells, MCF-7 cells, rat liver tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human prostate cancer tissue, human breast cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12922-3-AP targets HDAC2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HDAC2 fusion protein Ag3607|
|Full Name||histone deacetylase 2|
|Calculated molecular weight||458 aa, 52 kDa; 488 aa,55 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC031055|
|Gene ID (NCBI)||3066|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.
|Product Specific Protocols|
|WB protocol for HDAC2 antibody 12922-3-AP||Download protocol|
|IHC protocol for HDAC2 antibody 12922-3-AP||Download protocol|
|IF protocol for HDAC2 antibody 12922-3-AP||Download protocol|
|IP protocol for HDAC2 antibody 12922-3-AP||Download protocol|
|Click here to view our Standard Protocols|
Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells.
J Cell Mol Med
LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells.
Clin Transl Med
EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.
Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.
Cell Death Discov
Epigenetic derepression converts PPARγ into a druggable target in triple-negative and endocrine-resistant breast cancers.
Chin Med J (Engl)
Effect of Histone Deacetylase Inhibition on the Expression of Multidrug Resistance-associated Protein 2 in a Human Placental Trophoblast Cell Line.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (09-17-2020)
HDAC2 antibody is very good. Giving very clear bands and very good staining in tissue sections
Dipen (Verified Customer) (09-04-2020)
Excellent antibody. One single clean band at the correct molecular weight.