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  • KD/KO Validated

HDAC2 Antibody

HDAC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12922-3-AP

Synonyms

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human kidney tissue, Jurkat cells, MCF-7 cells, rat liver tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12922-3-AP targets HDAC2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag3607
Full Name histone deacetylase 2
Calculated molecular weight 582aa,65 kDa;488aa,55 kDa
Observed molecular weight 55-60 kDa
GenBank accession number BC031055
Gene symbol HDAC2
Gene ID (NCBI) 3066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Protocols

Product Specific Protocols
WB protocol for HDAC2 antibody 12922-3-APDownload protocol
IHC protocol for HDAC2 antibody 12922-3-APDownload protocol
IF protocol for HDAC2 antibody 12922-3-APDownload protocol
IP protocol for HDAC2 antibody 12922-3-APDownload protocol
FC protocol for HDAC2 antibody 12922-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells.

Authors - Huojun Zhang
humanchIP

J Cell Mol Med

LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells.

Authors - Ying Zhang
humanWB,IHC,IF

Clin Transl Med

EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.

Authors - Hongyu Zhao
  • KD Validated
humanWB

Chin Med J (Engl)

Effect of Histone Deacetylase Inhibition on the Expression of Multidrug Resistance-associated Protein 2 in a Human Placental Trophoblast Cell Line.

Authors - Hong-Yu Duan
mouse,ratWB

Chin Med J (Engl)

Disruption of Planar Cell Polarity Pathway Attributable to Valproic Acid-Induced Congenital Heart Disease through Hdac3 Participation in Mice.

Authors - Hong-Yu Duan
  • KD Validated
humanIHC

Oncol Lett

Clinical significance of enhancer of zeste homolog 2 and histone deacetylases 1 and 2 expression in peripheral T-cell lymphoma.

Authors - Huilai Zhang