HDAC2 Recombinant monoclonal antibody, PBS Only

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• HDAC2 Recombinant monoclonal antibody, PBS Only (81497-4-PBS)

HDAC2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 81497-4-PBS

Clone No.252017B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.5.1.-, EC:3.5.1.98, HD2, histone deacetylase 2, RPD3

• 

  WB analysis using 81497-4-RR (same clone as 81497-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81497-4-RR (HDAC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 81497-4-RR (same clone as 81497-4-PBS)

  IP result of anti-HDAC2 (IP:81497-4-RR, 4ug; Detection:81497-4-RR 1:800) with HEK-293 cells lysate 1080 ug. This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 81497-4-RR (same clone as 81497-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81497-4-RR (HDAC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 81497-4-RR (same clone as 81497-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81497-4-RR (HDAC2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 81497-4-RR (same clone as 81497-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (81497-4-RR, Clone: 252017B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 81497-4-RR (same clone as 81497-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC2 antibody (81497-4-RR, Clone: 252017B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 81497-4-RR (same clone as 81497-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (81497-4-RR, Clone: 252017B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 81497-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

81497-4-PBS targets HDAC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3607

  Product name: Recombinant human HDAC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 274-571 aa of BC031055

  Sequence: TDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVVKTFNLPLLMLGGGGYTIRNVARCWTHETAVALDCEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDAVHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSNP

• Full Name: histone deacetylase 2
• Calculated Molecular Weight: 458 aa, 52 kDa; 488 aa,55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC031055
• Gene Symbol: HDAC2
• Gene ID (NCBI): 3066
• RRID: AB_3743385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92769
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues, leading to the formation of a condensed and transcriptionally silenced chromatin. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3, and H4). At least 4 classes of HDAC were identified. As a class, I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression, and developmental events. This antibody is a rabbit monoclonal antibody raised against residues near the C terminus of human HDAC2.