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  • KD/KO Validated

HDAC4 Polyclonal antibody

HDAC4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 17449-1-AP

Synonyms

HA6116, HD4, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17449-1-AP targets HDAC4 in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC4 fusion protein Ag11572
Full Name histone deacetylase 4
Calculated molecular weight 972 aa, 106 kDa
Observed molecular weight 119-140 kDa
GenBank accession numberBC039904
Gene symbol HDAC4
Gene ID (NCBI) 9759
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.

Protocols

Product Specific Protocols
WB protocol for HDAC4 antibody 17449-1-APDownload protocol
IHC protocol for HDAC4 antibody 17449-1-APDownload protocol
IP protocol for HDAC4 antibody 17449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling.

Authors - Jianwei Li
humanWB

Diabetes Metab Syndr Obes

Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4.

Authors - Hang Zhao
  • KD Validated

Ann Clin Lab Sci

Expression of HDAC4 in Stage B Hepatocellular Carcinoma and Its Influence on Survival.

Authors - Zhonghong Wang
humanWB

J Cell Mol Med

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Authors - Shoujie Feng
  • KD Validated
humanWB

Front Genet

Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma.

Authors - Zezhong Mou
mouseWB,chIP

Biochim Biophys Acta

Exercise-induced GLUT4 transcription via inactivation of HDAC4/5 in mouse skeletal muscle in an AMPKα2-dependent manner.

Authors - Yanmei Niu