HDAC6 Monoclonal antibody

HDAC6 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (1)







Cat no : 67250-1-Ig


FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NCI-H1299 cells, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, SH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67250-1-Ig targets HDAC6 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HDAC6 fusion protein Ag28585
Full Name histone deacetylase 6
Calculated Molecular Weight 1063 aa, 114 kDa, 131 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC013737
Gene Symbol HDAC6
Gene ID (NCBI) 10013
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.


Product Specific Protocols
WB protocol for HDAC6 antibody 67250-1-IgDownload protocol
IHC protocol for HDAC6 antibody 67250-1-IgDownload protocol
IF protocol for HDAC6 antibody 67250-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Tissue Res

Upregulation of Profilin 2 on HDAC6 overexpression in mouse GC-1 cells and its putative role in germ cell migration in the testis

Authors - Pratibha Verma