HDDC2 Polyclonal antibody

HDDC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16632-1-AP

Synonyms

HCV NS5A-transactivated protein 2, EC:3.1.3.89, CGI-130, CGI 130, C6orf74

---

Validation Data Gallery

• 

  WB analysis using 16632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16632-1-AP (HDDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16632-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16632-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 16632-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16632-1-AP (HDDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, PC-3 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16632-1-AP targets HDDC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDDC2 fusion protein Ag9971
• Full Name: HD domain containing 2
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC003357
• Gene Symbol: HDDC2
• Gene ID (NCBI): 51020
• RRID: AB_10597248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.