HDDC3 Polyclonal antibody

HDDC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21091-1-AP

Synonyms

HD domain containing 3, HD domain containing protein 3, HDDC3, MESH1

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Validation Data Gallery

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  WB analysis using 21091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21091-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21091-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21091-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21091-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21091-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 21091-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21091-1-AP

  IP result of anti-HDDC3 (IP:21091-1-AP, 4ug; Detection:21091-1-AP 1:500) with HeLa cells lysate 3400ug.

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  IHC staining of human prostate cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, human heart tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue, A549 cells, rat heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human brain tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21091-1-AP is HDDC3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDDC3 fusion protein Ag15342
• Full Name: HD domain containing 3
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC033794
• Gene Symbol: HDDC3
• Gene ID (NCBI): 374659
• RRID: AB_10733121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679).

Publications

Species	Application	Title
human	WB	Cell Death Dis; MESH1 knockdown triggers proliferation arrest through TAZ repression.; Authors - Tianai Sun