HDGFRP3 Polyclonal antibody, PBS Only

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• HDGFRP3 Polyclonal antibody, PBS Only (12380-1-PBS)

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  WB analysis using 12380-1-AP (same clone as 12380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 12380-1-AP (same clone as 12380-1-PBS)

  IP result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12380-1-AP (same clone as 12380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12380-1-AP (HDGFRP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 12380-1-AP (same clone as 12380-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HDGFRP3 antibody (12380-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HDGFRP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12380-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HDGF-2, HDGF2, HDGF 2, CGI-142, CGI142

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Product Information

12380-1-PBS targets HDGFRP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3046

  Product name: Recombinant human HDGFRP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-203 aa of BC015483

  Sequence: MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT

• Full Name: hepatoma-derived growth factor, related protein 3
• Calculated Molecular Weight: 203 aa, 23 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015483
• Gene Symbol: HDGFRP3
• Gene ID (NCBI): 50810
• RRID: AB_2117222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y3E1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is a 30 kDa protein for phosphor modification.