HDHD2 Polyclonal antibody

HDHD2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26147-1-AP

Synonyms

3110052N05Rik, HDHD2

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Validation Data Gallery

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  WB analysis of mouse liver using 26147-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26147-1-AP (HDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26147-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26147-1-AP (HDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 26147-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 26147-1-AP (HDHD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26147-1-AP is HDHD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDHD2 fusion protein Ag23518
• Full Name: haloacid dehalogenase-like hydrolase domain containing 2
• Observed Molecular Weight: 18 kDa, 28 kDa
• GenBank Accession Number: BC033031
• Gene Symbol: HDHD2
• Gene ID (NCBI): 84064
• RRID: AB_2880404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.