HEBP2 Polyclonal antibody

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• HEBP2 Polyclonal antibody (12706-1-AP)

HEBP2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 12706-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

C6orf34, C6ORF34B, HEBP2, heme binding protein 2, KIAA1244, Placental protein 23, PP23, Protein SOUL, SOUL

• 

  WB analysis of human brain using 12706-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12706-1-AP (HEBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 12706-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12706-1-AP (HEBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 12706-1-AP

  IP result of anti-HEBP2 (IP:12706-1-AP, 3ug; Detection:12706-1-AP 1:500) with HEK-293 cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, human kidney tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12706-1-AP targets HEBP2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3387

  Product name: Recombinant human HEBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-205 aa of BC008205

  Sequence: MAEPLQPDPGAAEDAAAQAVETPGWKAPEDAGPQPGSYEIRHYGPAKWVSTSVESMDWDSAIQTGFTKLNSYIQGKNEKEMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFVRSFDGFSSAQKNQEQLLTLASILREDGKVFDEKVYYTAGYNSPVKLLNRNNEVWLIQKNEPTKENE

• Full Name: heme binding protein 2
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC008205
• Gene Symbol: HEBP2
• Gene ID (NCBI): 23593
• RRID: AB_2232981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Z4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEBP2(Heme-binding protein 2), also named SOUL and C6orf34, can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions (PMID: 17098234). It is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. hEBP2 is essential for the proliferation of human cells and the repression of hEBP2 severely decreases the ability of EBNA1 and EBV-based plasmids to bind mitotic chromosomes (PMID: 15923612).

Publications

What published studies show

The HEBP2 Polyclonal antibody (Cat# 12706-1-AP) has 1 citation. It was cited in Translational Oncology (IF 4.9, 2024) in a study on high-dimensional single-cell deconstruction of ovarian cancer. The research field encompasses ovarian cancer biology, tumor immune microenvironment (TIME) remodeling, and carboplatin resistance mechanisms. The antibody was applied in Western Blot (WB) for human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Transl Oncol; High-dimensional deconstruction of ovarian cancer at single-cell precision reveals HEBP2 that reshape the TIME and drive carboplatin resistance; Authors - BiKang Yang