HEC1/HEC/NDC80 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83859-1-RR (same clone as 83859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83859-1-RR (HEC1/HEC/NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR, Clone:5A2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

HEC1/HEC/NDC80 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83859-1-PBS

Clone No.5A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

NDC80, 5A2, HEC, HEC1, HsHec1

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Product Information

83859-1-PBS targets HEC1/HEC/NDC80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4220

  Product name: recombinant human HEC1/HEC protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 56-642 aa of NM_006101.3

  Sequence: LFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

• Full Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: NM_006101.3
• Gene Symbol: NDC80
• Gene ID (NCBI): 10403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O14777
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.