HECTD2 Polyclonal antibody

HECTD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25351-1-AP

Synonyms

HECT domain containing 2, HECTD2

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Validation Data Gallery

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  WB analysis of mouse brain using 25351-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25351-1-AP (HECTD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25351-1-AP is HECTD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HECTD2 fusion protein Ag18360
• Full Name: HECT domain containing 2
• Calculated Molecular Weight: 776 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC040187
• Gene Symbol: HECTD2
• Gene ID (NCBI): 143279
• RRID: AB_2880040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HECTD2(HECT domain-containing protein 2) may be an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein has 2 isoforms produced by alternative splicing.