HECW1 Polyclonal antibody

HECW1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24695-1-AP

Synonyms

HECW1, hNEDL1, KIAA0322, NEDL1

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Validation Data Gallery

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  IHC staining of human urothelial carcinoma using 24695-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 24695-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 24695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 24695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human urothelial carcinoma tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24695-1-AP is HECW1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HECW1 fusion protein Ag20264
• Full Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 1606 aa, 180 kDa
• GenBank Accession Number: BC151227
• Gene Symbol: HECW1
• Gene ID (NCBI): 23072
• RRID: AB_2918085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.