Tested Applications
| Positive WB detected in | MDA-MB-468 cells, A549 cells, U-118 MG cells |
| Positive IHC detected in | mouse cerebellum tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32026-1-AP targets HECW2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36316 Product name: Recombinant human HECW2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-650 aa of XM_017004563.1 Sequence: SFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVT Predict reactive species |
| Full Name | HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 |
| Calculated Molecular Weight | 176 kDa，1572aa |
| Observed Molecular Weight | 240 kDa |
| GenBank Accession Number | XM_017004563.1 |
| Gene Symbol | HECW2 |
| Gene ID (NCBI) | 57520 |
| RRID | AB_3742511 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9P2P5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HECW2 antibody 32026-1-AP | Download protocol |
| IHC protocol for HECW2 antibody 32026-1-AP | Download protocol |
| WB protocol for HECW2 antibody 32026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HECW2 Polyclonal antibody (Cat# 32026-1-AP) has 1 citation. It was cited in Advanced Science (2026) in a study on psychological stress-associated bile acid reprogramming promoting hepatocellular carcinoma progression. The research field is liver cancer (hepatocellular carcinoma) and bile acid metabolism. The antibody was used in Western Blot (WB) on human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Psychological Stress Associated Bile Acid Reprogramming Promotes Hepatocellular Carcinoma Progression |
Reviews
What customers say
The single review rates this antibody 4 out of 5 stars. It was used for Western Blotting on human lung cancer cell lines at a 1:1000 dilution with overnight incubation. The reviewer noted the band was initially faint but improved with longer exposure, ultimately producing a good image. Overall, the reviewer recommends this antibody for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikolett (Verified Customer) (09-25-2025) | This antibody is working on human lung cancer cell lines for Western Blotting after overnight incubation. The band was a bit faint but after exposure improvements I achieved a nice image. I recommend this antibody.
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