Tested Applications
| Positive WB detected in | MDA-MB-468 cells, A549 cells, U-118 MG cells |
| Positive IHC detected in | mouse cerebellum tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32026-1-AP targets HECW2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36316 Product name: Recombinant human HECW2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-650 aa of XM_017004563.1 Sequence: SFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVT Predict reactive species |
| Full Name | HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 |
| Calculated Molecular Weight | 176 kDa，1572aa |
| Observed Molecular Weight | 240 kDa |
| GenBank Accession Number | XM_017004563.1 |
| Gene Symbol | HECW2 |
| Gene ID (NCBI) | 57520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9P2P5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HECW2 antibody 32026-1-AP | Download protocol |
| IHC protocol for HECW2 antibody 32026-1-AP | Download protocol |
| WB protocol for HECW2 antibody 32026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nikolett (Verified Customer) (09-25-2025) | This antibody is working on human lung cancer cell lines for Western Blotting after overnight incubation. The band was a bit faint but after exposure improvements I achieved a nice image. I recommend this antibody.
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