HELIOS Recombinant monoclonal antibody

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• HELIOS Recombinant monoclonal antibody (87876-1-RR)

• 

  WB analysis using 87876-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87876-1-RR (HELIOS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 87876-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HELIOS antibody (87876-1-RR, Clone: 253388A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HELIOS Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87876-1-RR

Clone No.253388A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

IKZF2, IKZF 2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells, EL-4 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87876-1-RR targets HELIOS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4087

  Product name: Recombinant human IKZF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 227-526 aa of BC028936

  Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH

• Full Name: IKAROS family zinc finger 2 (Helios)
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC028936
• Gene Symbol: HELIOS
• Gene ID (NCBI): 22807
• RRID: AB_3745802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKS7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HELIOS, encoded by the IKZF2 gene (Ikaros Family Zinc Finger Protein 2), is a transcription factor belonging to the Ikaros family of zinc-finger proteins. It is primarily expressed in hematopoietic cells, particularly in regulatory T cells (Tregs) and early lymphoid progenitors, where it plays a critical role in immune regulation, T-cell development, and the maintenance of Treg suppressive function. HELIOS binds to specific DNA sequences to regulate gene expression involved in lymphocyte differentiation and immune homeostasis. It is also widely used as a stable marker for activated Tregs. Dysregulation of HELIOS has been implicated in autoimmune diseases, allergy, and cancer immunotherapy resistance.