Tested Applications
| Positive WB detected in | K-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse thymus tissue, mouse testis tissue, rat testis tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11955-1-AP targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2558 Product name: Recombinant human HELLS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC031004 Sequence: MFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF Predict reactive species |
| Full Name | helicase, lymphoid-specific |
| Calculated Molecular Weight | 348 aa, 40 kDa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC031004 |
| Gene Symbol | HELLS |
| Gene ID (NCBI) | 3070 |
| RRID | AB_2117529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HELLS antibody 11955-1-AP | Download protocol |
| IF protocol for HELLS antibody 11955-1-AP | Download protocol |
| IHC protocol for HELLS antibody 11955-1-AP | Download protocol |
| IP protocol for HELLS antibody 11955-1-AP | Download protocol |
| WB protocol for HELLS antibody 11955-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HELLS Polyclonal antibody (Cat# 11955-1-AP) has 21 citations published in journals including Nature Genetics, Nature Communications, Nucleic Acids Research, Science Advances, Cell Reports, Oncogenesis, Advanced Science, Genome Biology, and Human Molecular Genetics, among others. Research fields span epigenetics and DNA methylation, chromatin remodeling, centromere biology, B-cell development, oocyte aging, ferroptosis, EMT, and multiple cancer types (hepatocellular, gastric, pancreatic, prostate, nasopharyngeal, liver). Cited applications include WB, IF, CoIP, ChIP, IP, and IHC in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Commun Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells | ||
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Nucleic Acids Res Photoclick chemistry led to the identification of HELLS as a helicase for DNA G-quadruplexes.
| ||
Nucleic Acids Res The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression | ||
Adv Sci (Weinh) Multi-Omics Analysis Reveals Translational Landscapes and Regulations in Mouse and Human Oocyte Aging |
Reviews
What customers say
Both reviewers gave 5-star ratings. One user tested the antibody for WB and IF in mouse testis, GC1, and NIH3T3 cells, reporting specific nuclear staining with low background in IF and an expected ~100 kDa band in WB, though some minor non-specific bands were observed. The other user used it for WB in HEK293, Jurkat, HAP1, and PBMC samples, praising its performance for HELLS detection and noting that multiple isoform bands appeared across cell lines. Both appreciated the provided protocols and responsive customer support during troubleshooting.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Diana (Verified Customer) (08-18-2022) | In IF the antibody binds specific in the nucleus and has low background, thats great! In WB I have a band as aspected around 100kd but I also have some other smaller unspecific bands. Here I will work on the troubleshooting. Thanks for the corresponding protocolls with buffers and incubation time.
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Michelle (Verified Customer) (10-24-2019) | Great antibody for HELLS. Customer support was really helpful when I was troubleshooting how to clean up my western blot. Changed the path of my project since multiple bands for different isoforms of the protein appeared in the cell lines I was invesitgating.
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