HELLS Recombinant monoclonal antibody

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  WB analysis using 87169-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87169-1-RR (HELLS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity of 87169-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87169-1-RR against Human HELLS were performed. The affinity constant is 0.749 nM.

HELLS Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87169-1-RR

Clone No.252319G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.6.4.-, helicase, lymphoid, LSH, Lymphoid specific helicase, Lymphoid-specific helicase

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, PC-3 cells, NIH/3T3 cells, mouse thymus tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87169-1-RR targets HELLS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2558

  Product name: Recombinant human HELLS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-348 aa of BC031004

  Sequence: MFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF

• Full Name: helicase, lymphoid-specific
• Calculated Molecular Weight: 348 aa, 40 kDa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC031004
• Gene Symbol: HELLS
• Gene ID (NCBI): 3070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRZ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.