Anti-Human HER2/ErbB2 Rabbit Recombinant Antibody

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• Anti-Human HER2/ErbB2 Rabbit Recombinant Antibody (98304-3-RR)

HER2/ErbB2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98304-3-RR

Clone No.242436C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ERBB2, CD340, HER 2, HER 2/neu, HER2

• 

  FC experiment of SK-BR-3 using 98304-3-RR

  1x10^6 SK-BR-3 cells (left) or MCF-7 cells (right) were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98304-3-RR

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98304-3-RR

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98304-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98304-3-RR against Human HER2/ErbB2 were performed. The affinity constant is 3.53 nM.

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Tested Applications

• Positive FC detected in: SK-BR-3 cells, MCF-7 cells, Transfected CHO

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98304-3-RR targets HER2/ErbB2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Calculated Molecular Weight: 1255 aa, 138 kDa
• GenBank Accession Number: BC156755
• Gene Symbol: HER2/ErbB2
• Gene ID (NCBI): 2064
• RRID: AB_3745964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04626
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.