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HERC4 Polyclonal antibody, PBS Only (13691-1-PBS)

Filter: AllWBIPIF/ICC

WB analysis of human brain using 13691-1-AP (same clone as 13691-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
WB analysis using 13691-1-AP (same clone as 13691-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
WB analysis using 13691-1-AP (same clone as 13691-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
WB analysis using 13691-1-AP (same clone as 13691-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 13691-1-AP (same clone as 13691-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 13691-1-AP (same clone as 13691-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 13691-1-AP (same clone as 13691-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 13691-1-AP (same clone as 13691-1-PBS)
IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 13691-1-AP (same clone as 13691-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

HERC4 Polyclonal antibody, PBS Only

Name: HERC4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13691-1-PBS

HERC4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 13691-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.3.2.26, HECT domain and RCC1-like domain-containing protein 4, hect domain and RLD 4, HECT-type E3 ubiquitin transferase HERC4, KIAA1593

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13691-1-PBS targets HERC4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4566 Product name: Recombinant human HERC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 723-1049 aa of BC039600 Sequence: NIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI Predict reactive species
Full Name	hect domain and RLD 4
Calculated Molecular Weight	1049 aa, 118 kDa
Observed Molecular Weight	107 kDa
GenBank Accession Number	BC039600
Gene Symbol	HERC4
Gene ID (NCBI)	26091
RRID	AB_10596480
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5GLZ8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)