|Positive WB detected in||HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells|
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11317-1-AP targets HEXA in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HEXA fusion protein Ag1854|
|Full Name||hexosaminidase A (alpha polypeptide)|
|Calculated molecular weight||529 aa, 60 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC018927|
|Gene ID (NCBI)||3073|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).
The phagosomal proteome in interferon-gamma-activated macrophages.
VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation.
Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.
Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis
Proc Natl Acad Sci U S A
A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB
Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joshua (Verified Customer) (01-26-2023)
Did not detect expected mature HEXA ~54kDa.
Georgina (Verified Customer) (09-15-2022)
Good to have the chance to buy a sample to try out an experiment. The antibody worked.