HEXA Polyclonal antibody

HEXA Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 29259-1-AP

Synonyms

HEXA, Hexosaminidase subunit A, TSD



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29259-1-AP targets HEXA in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEXA fusion protein Ag30808
Full Name hexosaminidase A (alpha polypeptide)
Calculated molecular weight 529 aa, 60 kDa
Observed molecular weight 50-54 kDa, 60-67 kDa
GenBank accession numberBC018927
Gene symbol HEXA
Gene ID (NCBI) 3073
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

Protocols

Product Specific Protocols
WB protocol for HEXA antibody 29259-1-APDownload protocol
IHC protocol for HEXA antibody 29259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols