Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, mouse kidney tissue, Jurkat cells, HeLa cells, mouse lung tissue, rat kidney tissue
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16229-1-AP targets HEXB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|HEXB fusion protein Ag8683
|hexosaminidase B (beta polypeptide)
|Calculated molecular weight
|556 aa, 63 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).
The Role of Oxidized Cholesterol in Diabetes-Induced Lysosomal Dysfunction in the Brain.
Front Cell Dev Biol
Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.
Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.
Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo.
Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis
J Cereb Blood Flow Metab
Characteristics of activation of monocyte-derived macrophages versus microglia after mouse experimental intracerebral hemorrhage