|Positive WB detected in||mouse testis tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human brain tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19929-1-AP is HEY1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HEY1 fusion protein Ag13906|
|Full Name||hairy/enhancer-of-split related with YRPW motif 1|
|Calculated molecular weight||304 aa, 33 kDa|
|Observed molecular weight||32-34 kDa|
|GenBank accession number||BC001873|
|Gene ID (NCBI)||23462|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.
Biochem Cell Biol
MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.
Undifferentiated spermatogonia regulate Cyp26b1 expression through NOTCH signaling and drive germ cell differentiation.
A Noncoding Regulatory RNAs Network Driven by Circ-CDYL Acts Specifically in the Early Stages Hepatocellular Carcinoma.
Mol Med Rep
Salidroside suppresses the metastasis of hepatocellular carcinoma cells by inhibiting the activation of the Notch1 signaling pathway.
2B.06: EPIGALLOCATECHIN-3-GALLATE ATTENUATES URIC ACID-INDUCED INFLAMMATORY RESPONSES AND OXIDATIVE STRESS BY MODULATING NOTCH PATHWAY.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Zhenminm (Verified Customer) (07-10-2019)
It is too much non-specific background.