HEY2 Recombinant monoclonal antibody, PBS Only

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  IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83501-7-RR (same clone as 83501-7-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83501-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

HEY2 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83501-7-PBS

Clone No.240474F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

240474F2, bHLHb32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32

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Product Information

83501-7-PBS targets HEY2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30988

  Product name: Recombinant human HEY2 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-337 aa of BC007707

  Sequence: MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF

• Full Name: hairy/enhancer-of-split related with YRPW motif 2
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC007707
• Gene Symbol: HEY2
• Gene ID (NCBI): 23493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UBP5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system.