HEYL Polyclonal antibody

HEYL Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15679-1-AP

Synonyms

hHeyL, HEY3, Hairy-related transcription factor 3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33

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Validation Data Gallery

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  WB analysis using 15679-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15679-1-AP (HEYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 15679-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15679-1-AP (HEYL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15679-1-AP

  IP result of anti-HEYL (IP:15679-1-AP, 4ug; Detection:15679-1-AP 1:300) with HeLa cells lysate 2600ug.

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  IF Staining of HeLa using 15679-1-AP

  Immunofluorescent analysis of Hela cells, using HEYL antibody 15679-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

15679-1-AP targets HEYL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEYL fusion protein Ag8163
• Full Name: hairy/enhancer-of-split related with YRPW motif-like
• Calculated Molecular Weight: 328 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC006087
• Gene Symbol: HEYL
• Gene ID (NCBI): 26508
• RRID: AB_2878167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEYL, also named as Hairy/enhancer-of-split related with YRPW motif-like protein, is a 328 amino acid protein, which belongs to the HEY family. HEYL as downstream effector of Notch signaling may be required for cardiovascular development. HEYL represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner; Authors - Hao Sun