HHIP Polyclonal antibody

HHIP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 11654-1-AP

Synonyms

hedgehog interacting protein, HHIP, HIP

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 11654-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11654-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11654-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11654-1-AP (HHIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11654-1-AP targets HHIP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HHIP fusion protein Ag2241
• Full Name: hedgehog interacting protein
• Calculated Molecular Weight: 700 aa, 79 kDa
• GenBank Accession Number: BC025311
• Gene Symbol: HHIP
• Gene ID (NCBI): 64399
• RRID: AB_2232921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HHIP is a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.

Publications

Species	Application	Title
mouse	WB	J Mol Cell Biol; Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.; Authors - Yuwei Li
human	WB	Int Immunopharmacol; Wogonin inhibits the growth of HT144 melanoma via regulating hedgehog signaling-mediated inflammation and glycolysis.; Authors - Ling Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chaoqun (Verified Customer) (07-30-2019)

For IF analysis of paraffin embedded mouse lung section, it did not work well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Paraffin embedded mouse lung section