HIC1 Polyclonal antibody

HIC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24949-1-AP

Synonyms

hic 1, HIC1, hypermethylated in cancer 1, ZBTB29



Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24949-1-AP is HIC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIC1 fusion protein Ag21849
Full Name hypermethylated in cancer 1
Calculated molecular weight 714 aa, 75 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC157027
Gene symbol HIC1
Gene ID (NCBI) 3090
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIC1 antibody 24949-1-APDownload protocol
IHC protocol for HIC1 antibody 24949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols