HIC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HIC2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22788-1-AP

Synonyms

Hic 2, Hic 3, HIC2, HRG22, hypermethylated in cancer 2, KIAA1020, ZBTB30

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Validation Data Gallery

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  WB analysis of Jurkat using 22788-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22788-1-AP (HIC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22788-1-AP

  IP result of anti-HIC2 (IP:22788-1-AP, 4ug; Detection:22788-1-AP 1:300) with Jurkat cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

22788-1-AP targets HIC2 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIC2 fusion protein Ag18789
• Full Name: hypermethylated in cancer 2
• Calculated Molecular Weight: 615 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC094787
• Gene Symbol: HIC2
• Gene ID (NCBI): 23119
• RRID: AB_2879169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumour suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).

Publications

Species	Application	Title
human	WB	Nat Genet; HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription; Authors - Peng Huang ; KD Validated
human	WB	Blood; Let-7 miRNAs repress HIC2 to regulate BCL11A transcription and hemoglobin switching; Authors - Peng Huang
human	WB	Am J Cancer Res; Small extracellular vesicle-associated miR-6068 promotes aggressive phenotypes of prostate cancer through miR-6068/HIC2/SIRT1 axis; Authors - Mohamed S A Gaballah